Cell Line Authentication – NIH Requirements

The NIH has recently started requiring researchers to authenticate their key biological and chemical resources for any NIH funded work.  In addition, many journals are also requiring authentication prior to accepting manuscripts for publication.  The DNA Sequencing Core has brought on testing to authenticate human cell lines used in research on campus.  The standard is currently evolving and is likely to do so for some time, but currently 8 loci tested by STR is what is required.  We have brought on a kit that tests these 8 plus others (to a total of 24 loci).  This kit tests the same loci used in American and European forensics work.

To run your samples through the core, you need to submit at least 10uL of 10ng/uL extracted DNA from your cell line.  Cost is currently $40/cell line.  The test is run on an as needed basis currently no more than one time per week.  After your cell line is run, you will receive genotype calls on the loci that you can compare to online databases.  If it is your first time, please contact Derek Warner, director, to assist you on how to query the databases so that you can confirm your cell line identity.  For more information, please visit the core website.

For additional questions, please contact Derek Warner at (801) 581-4736 or dwarner@cores.utah.edu.

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